Hek293 Transfection Reagent. [tab:Products] EndoFectin™ Max Transfection Reagent EndoFect

[tab:Products] EndoFectin™ Max Transfection Reagent EndoFectin™ Max is a lipid-based transfection Low transfection efficiency is a multifaceted issue that can stem from several factors. They serve as a versatile platform for scientific investigations and the production of biological products. 5mL vial TransIT-Lenti Transfection , enhance packaging and transfer vectors to adherent HEK 293 cell types to increase recombinant lentivirus production. High efficiency delivering both DNA and RNA into HEK293 cells Explore transfection of HEK293 cells and compare efficiency of commercial transfection reagents. Transfection reagent for HEK293 cellline, a human epithelial kidney cells. Use this procedure to transfect plasmid DNA into HEK 293 cells in a 24-well format (for other formats, see Scaling up or down transfections, below). The adenoviral genes expressed in this cell line allow the cells to produce very high levels of recombinant proteins. . All amounts and volumes are given on a per well basis. The most critical aspects to evaluate are the health and confluency of your cells, the quality and quantity of your plasmid DNA, the choice and optimization of the transfection reagent, and the presence of any inhibitors in your culture system. The major advantages of 293Tran include: 293-Free™ Transfection Reagent Animal-free polycationic liposomal transfection reagent optimized for the transfection of HEK293 cells grown in suspension culture. [1] RNA interference (RNAi) is a powerful technique for sequence-specific gene silencing initiated by short interfering RNA (siRNA). Propriedades Nome do produto 293-Free Transfection Reagent, Animal-free polycationic liposomal transfection reagent optimized for the transfection of HEK293 cells grown in suspension culture. X-tremeGENE siRNA Transfection Reagent efficiently delivers short interfering RNA (siRNA) into many commonly used cell types including HeLa, NIH 3T3, HEK-293, CHO-K1, and COS-7, and several hard-to-transfect cell lines, such as HT29, a human adenocarcinoma cell line. TransIT-Lenti Transfection Reagent, 1. Learn about HEK293 transfection protocols and cationic lipid methods. TransIT-293 Transfection Reagent is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in HEK 293 and cell types of associated lineage. Aug 28, 2025 · Human Embryonic Kidney 293 (HEK293) cells are a widely used cell line in biological research and biotechnology. The successful delivery of siRNA into cells is a critical step for achieving significant X-tremeGENE™ siRNA Transfection Reagent efficiently introduces siRNA into cells for gene knockdown experiments in both common and hard-to-transfect cell lines. See below to learn more about. A versatile & efficient suite of robust transfection reagents GeneCopoeia's EndoFectin™ line of transfection reagents provide affordable and powerful solutions that encompass a range of different applications, general purpose reagents like EndoFectin™ Max. Polyethylenimine (PEI) with an average molecular weight of 40kDa is one of the most widely used cationic polymer based transfection reagents. Incubation: Incubate the cells at 37°C with 5% CO2 for 24-48 hours before proceeding with the BI-749327 treatment. its high water solubility enables effcient binding to negatively charged nucleic acids forming stable PEI-DNA complexes that facilitate endocytosis through interactions with the cell membrane. They are also known, more informally, as HEK cells. 3 days ago · HEK293 viral vector production is highly sensitive to upstream variables, and Cell Culture Flask quality is one of the most underappreciated factors. Transfection: Add 50 μL of the transfection complex to each well. 4 days ago · HEK293 cells are Human Embryonic Kidney cells, originally isolated and grown by Dutch biologist Alex Van der Eb in the early 1970s. The cells were established by a Dutch biologist named Alex Van der Eb in the early 1970s. The HEK293 cell line is a permanent line established from primary embryonic human kidney, which was transformed with sheared human adenovirus type 5 DNA. The use of 293 refers to the experiment number referenced in his laboratory note book. Among the human cell lines, HEK293 is widely utilized due to its high transfectivity, rapid growth rate, and ability to grow in a serum-free, suspension culture. Product features• Low toxicity: combined with high It is design for the transfection of a viral particle solution composed by either one viral vector or vectors encoding cas9 and sgRNA. The 1 ml size provides enough reagent to transfect a liter of culture. 2018 To validate Lipid 10, its performance was benchmarked against two widely used commercial reagents: LipofectamineTM 3000 and FuGENE® HD. They were transfected with sheared adenovirus 5 (Ad5) DNA by Frank Graham, a postdoc in Van der Eb’s lab. Use this procedure to transfect plasmid DNA into HEK 293 cells in a 24-well format (for other formats, see Scaling up or down transfections, below).

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